细胞名称 J1小鼠胚胎干细胞J1
Cell Name
描述 小鼠全能性胚胎干细胞，每支细胞量1×106。 
动物种别 小鼠，129S4/SvJae品系
Organism
组织来源 小鼠胚胎内细胞团
Tissue and Cell Type
形态 球形克隆
Morphology
培养基和添加剂 mES完全培养液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES级FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（终浓度为1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
Complete Growth Medium and Culture Conditions
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Stevens LC. A new inbred subline of mice (129-terSv) with a high incidence of spontaneous congenital testicular teratomas. J. Natl. Cancer Inst. 50: 235-242, 1973. PubMed: 4692863 Li E, et al. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69: 915-926, 1992. PubMed: 1606615 Krzyzanowski PM, et al. Identification of novel stem cell markers using gap analysis of gene expression data. Genome Biology 8(9): R193, 2007. PubMed: 17875203 Luikenhuis S, et al. Antisense transcription through the Xist locus mediates Tsix function in embryonic stem cells. Mol. Cell Biol. 21(24): 8512?8520, 2001. PubMed: 11713286 Goto T, et al. Regulation of X-Chromosome inactivation in development in mice and humans. Micro. Molec. Biol. Rev. (ASM) 62(2): 362-378, 1998. PubMed: 9618446 Yap DYL, et al. Using biomarker signature patterns for an mRNA molecular diagnostic of mouse embryonic stem cell differentiation state. BMC Genomics 8: 210, 2007. PubMed: 17605829 Haase VH, et al. Vascular tumors in livers with targeted inactivation of the von Hippel?Lindau tumor suppressor. Proc. Natl. Acad. Sci. 98(4): 1583?1588, 2001. PubMed: 11171994 Oda M, et al. DNA methylation regulates long-range gene silencing of an X-linked homeobox gene cluster in a lineage-specific manner. Genes Dev. 20(24): 3382-3394, 2006. PubMed: 17182866 Lorincz MC, et al. DNA methylation density influences the stability of an epigenetic imprint and Dnmt3a/b-independent de novo methylation. Mol. Cell Biol. 22(21): 7572?7580, 2002.PubMed: 12370304 Tucker KL, et al. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res. 25: 3745-3746, 1997. PubMed: 9278500 Biniszkiewicz D, et al. Dnmt1 overexpression causes genomic hypermethylation, loss of imprinting, and embryonic lethality. Mol. Cell Biol. 22(7): 2124-2135, 2002. PubMed: 11884600

运输和保存

可选择干冰运输及发送复苏存活细胞方式：

（1）干冰运输，收到后立即转入液氮冻存或直接复苏；

（2）存活细胞，收到后应继续生长，传代达到细胞生长状态良好时，再进行冻存。具体操作见细胞培养步骤。
细胞用途：仅供科研使用。

注意事项