细胞名称 PC-3人前列腺癌细胞
Cell Name
描述 人前列腺癌细胞
Description
动物种别 人
Organism
组织来源 前列腺
Tissue and Cell Type
形态 上皮样
Morphology
培养基和添加剂 1. 人前列腺癌细胞PC-3细胞完全培养液配方(100 ml):
Complete Growth Medium and Culture Conditions Ham's F-12K (Kaighn's) Medium (Invitrogen, 21127-022) 90 ml
FBS (Gibco) 10 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Kaighn ME, et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. PubMed: 447482 Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet. Cell Genet. 62: 183-184, 1993. PubMed: 8428522 Ohnuki Y, et al. Chromosomal analysis of human prostatic adenocarcinoma cell lines. Cancer Res. 40: 524-534, 1980. PubMed: 7471073 Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194 Umekita Y, et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218 Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628 Nupponen NN, et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 9460501 Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Su ZZ, et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 8692978
运输和保存
可选择干冰运输及发送复苏存活细胞方式:
(1)干冰运输,收到后立即转入液氮冻存或直接复苏;
(2)存活细胞,收到后应继续生长,传代达到细胞生长状态良好时,再进行冻存。具体操作见细胞培养步骤。
细胞用途:仅供科研使用。
注意事项
地址:上海一研生物科技有限公司
Shanghai yiyan bio-technology Co. Ltd.
电话:15021460884
电话:13611928337
电话:021-69985186
电话:021-69985169
网址:www.E-research.com.cn
本公司的所有产品仅用于科学研究或者工业科研等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。