细胞名称 CHO-K1中国仓鼠卵巢细胞亚株
Cell Name
描述 中国仓鼠卵巢细胞亚株
Description
动物种别 中国仓鼠
Organism
组织来源 卵巢
Tissue and Cell Type
形态 上皮样
Morphology
培养基和添加剂 F-12K（Invitrogen，21127-022）+10%FBS (Gibco)
Complete Growth Medium and Culture Conditions 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821 Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058 Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed:5244736 Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed:6068403 Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351 Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752 Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365 Hoppe HC, et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. PubMed: 9284169 Aragon V, et al. Effect of cytolethal distending toxin on F-actin assembly and cell division in Chinese hamster ovary cells. Infect. Immun. 65: 3774-3780, 1997. PubMed: 9284151 Yang TT, et al. Quantification of gene expression with a secreted alkaline phosphatase reporter system. BioTechniques 23: 1110-1114, 1997. PubMed: 9421645 St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Siess DC, et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675 Wang H, et al. Modulation of ecotropic murine retrovirus by N-linked glycosylation of the cell surface receptor/amino acid transporter. J. Virol. 70: 6884-6891, 1996. PubMed: 8794331 Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed:8971005 Nohturfft A, et al. Recurrent G-to-A substitution in a single codon of SREBP cleavage-activating protein causes sterol resistance in three mutant chinese hamster ovary cell lines. Proc. Natl. Acad. Sci. USA 93: 13709-13714, 1996. PubMed: 8942999 Blanke SR, et al. Fused polycationic peptide mediates delivery on diphtheria toxin A chain to the cytosol in the presence of anthrax protective antigen. Proc. Natl. Acad. Sci. USA 93: 8437-8442, 1996. PubMed: 8710889 Efthimiopoulos S, et al. Cholinergic agonists stimulate secretion of soluble full-length amyloid precursor protein in neuroendocrine cells. Proc. Natl. Acad. Sci. USA 93: 8046-8050, 1996. PubMed:8755600 Sharma A, et al. Reduction in the level of Gal(alpha 1,3)Gal in transgenic mice and pigs by the expression of an alpha(1,2)fucosyltransferase. Proc. Natl. Acad. Sci. USA 93: 7190-7195, 1996. PubMed: 8692967 Ozcelebi F, et al. Phosphorylation of cholecystokinin receptors expressed on chinese hamster ovary cells. J. Biol. Chem. 271: 3750-3755, 1996. PubMed: 8631990 Lee RJ, Huang L. Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer. J. Biol. Chem. 271: 8481-8487, 1996. PubMed: 8626549 Hawes BE, et al. Phosphatidylinositol 3-kinase is an early intermediate in the G beta gamma-mediated mitogen-activated protein kinase signaling pathway. J. Biol. Chem. 271: 12133-12136, 1996. PubMed: 8647803 Parker A, et al. Identification of the extracellular matrix binding sites for insulin-like growth factor-binding protein 5. J. Biol. Chem. 271: 13523-13529, 1996. PubMed: 8662813 Beving DE, et al. Oral immunization with a recombinant cysteine-rich section of the Entamoeba histolytica galactose-inhibitable lectin elicits an intestinal secretory immunoglobulin A response that has in vitro adherence inhibition activity. Infect. Immun. 64: 1473-1476, 1996. PubMed: 8606122 Holtmann MH, et al. Role of receptor phosphorylation in desensitization and internalization of the secretin receptor. J. Biol. Chem. 271: 23566-23571, 1996. PubMed: 8798566 Lugemwa FN, et al. Unusual beta-D-xylosides that prime glycosaminoglycans in animal cells. J. Biol. Chem. 271: 19159-19165, 1996. PubMed: 8702593

运输和保存

可选择干冰运输及发送复苏存活细胞方式：

（1）干冰运输，收到后立即转入液氮冻存或直接复苏；

（2）存活细胞，收到后应继续生长，传代达到细胞生长状态良好时，再进行冻存。具体操作见细胞培养步骤。
细胞用途：仅供科研使用。

注意事项