细胞名称 C2C12小鼠胚胎肌母细胞
Cell Name
描述 小鼠胚胎肌母细胞
Description
动物种别 小鼠 品系C3H
Organism
组织来源 肌肉
Tissue and Cell Type
形态 肌母细胞状
Morphology
培养基和添加剂 DMEM Medium (Invitrogen, 12430-054) 89 ml
Complete Growth Medium and Culture Conditions FBS (Gibco) 10 ml
Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524 Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846 Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324 Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336 Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 Qing Y, et al. Inhibitory effects of iron on bone morphogenetic protein 2-induced osteoblastogenesis. J. Bone Miner. Res. 26(6): 1188-1196, 2011. PubMed: 21308772
运输和保存
可选择干冰运输及发送复苏存活细胞方式:
(1)干冰运输,收到后立即转入液氮冻存或直接复苏;
(2)存活细胞,收到后应继续生长,传代达到细胞生长状态良好时,再进行冻存。具体操作见细胞培养步骤。
细胞用途:仅供科研使用。
注意事项
地址:上海一研生物科技有限公司
Shanghai yiyan bio-technology Co. Ltd.
电话:15021460884
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电话:021-69985186
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本公司的所有产品仅用于科学研究或者工业科研等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。