细胞名称 NIH/3T3小鼠胚胎成纤维细胞
Cell Name
描述 小鼠胚胎成纤维细胞
Description
动物种别 小鼠，品系NIH/Swiss
Organism
组织来源 胚胎
Tissue and Cell Type
形态 成纤维细胞
Morphology
培养基和添加剂 DMEM (Invitrogen, 12430) 87 ml
Complete Growth Medium and Culture Conditions 小牛血清bovine calf serum (四季青) 10 ml
Glutamax（invitrogen 35050） 1 ml
Non-essential Amino Acids, 100×(Invitrogen, 11140) 1 ml
Sodium Pyruvate 100 mM Solution（invitrogen 11360070） 1 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Jainchill JL, et al. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J. Virol. 4: 549-553, 1969. PubMed: 4311790 Andersson P, et al. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Cell 16: 63-75, 1979. PubMed:84715 Copeland NG, Cooper GM. Transfection by exogenous and endogenous murine retrovirus DNAs. Cell 16: 347-356, 1979. PubMed: 222457 Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256 Jones PL, et al. Tumor necrosis factor alpha and interleukin-1beta regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C/EBP-beta and NF-kappaB. Mol. Cell. Biol. 17: 6970-6981, 1997. PubMed:9372929 Gonzalez Armas JC, et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915 Siess DC, et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675 Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Medin JA, et al. Correction in trans for Fabry disease: expression, secretion, and uptake of alpha-galactosidase A in patient-derived cells driven by a high-titer recombinant retroviral vector. Proc. Natl. Acad. Sci. USA 93: 7917-7922, 1996. PubMed: 8755577 Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Cranmer LD, et al. Identification, analysis, and evolutionary relationships of the putative murine cytomegalovirus homologs of the human cytomegalovirus UL82 (pp71) and UL83 (pp65) matrix phosphoproteins. J. Virol. 70: 7929-7939, 1996. PubMed:8892916 Shisler J, et al. Induction of susceptibility to tumor necrosis factor by E1A is dependent on binding to either p300 or p105-Rb and induction of DNA synthesis. J. Virol. 70: 68-77, 1996. PubMed:8523594 Cavanaugh VJ, et al. Murine cytomegalovirus with a deletion of genes spanning HindIII-J and -I displays altered cell and tissue tropism. J. Virol. 70: 1365-1374, 1996. PubMed: 8627652 Westerman KA, Leboulch P. Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc. Natl. Acad. Sci. USA 93: 8971-8976, 1996. PubMed: 8799138 The NIH/3T3, a continuous cell line of highly contact-inhibited cells was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 (ATCC CCL-92) and the inbred BALB/c 3T3 (ATCC CCL-163). The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays.

运输和保存

可选择干冰运输及发送复苏存活细胞方式：

（1）干冰运输，收到后立即转入液氮冻存或直接复苏；

（2）存活细胞，收到后应继续生长，传代达到细胞生长状态良好时，再进行冻存。具体操作见细胞培养步骤。
细胞用途：仅供科研使用。

注意事项