细胞名称 OP9小鼠胚胎干细胞OP9
Cell Name
描述 小鼠胚胎干细胞 OP9,每支细胞量1×106。 
Description 细胞说明书可至网盘（https://yunpan.cn/cPYPmJixDueRW 访问密码 3956）下载
动物种别 小鼠，品系(C57BL/6 x C3H)F2 -op/op
Organism
组织来源 骨髓
Tissue and Cell Type
形态 fibroblast-like
Morphology
培养基和添加剂 小鼠胚胎干细胞OP9完全培养液配方（100 ml）：
Complete Growth Medium and Culture Conditions α-MEM（invitrogen 12561） 80 ml
ES级FBS（Gibco） 20 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
备注：保持细胞密度在4 x 103 ~ 1 x 104 /cm2 之间，不可过密或过稀
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Brook FA, et al. The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome. Diabetes. 52:205-208, 2003. PubMed: 12502514 Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. USA. 94: 5709-5712, 1997. PubMed: 9159137 Schuster-Gossler K, et al. Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines. BioTechniques 31: 1022-1024, 1026, 2001. PubMed: 11730008 Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865 Matise MP, et al. Production of targeted Embryonic Stem Cell Clones. In AL Joyner (Ed.), Gene Targeting: A Practical Approach. Oxford University Press, Oxford, p. 101-132, 1999. Lemckert FA, et al. Gene targeting in C57BL/6 ES cells. Successful germ line transmission using recipient BALB/c blastocysts developmentally matured in vitro. Nucleic Acids Res. 25: 917-918, 1997. PubMed: 9016649 Ledermann B, Burki K. Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Exp. Cell Res. 197: 254-258, 1991. PubMed: 1959560

运输和保存

可选择干冰运输及发送复苏存活细胞方式：

（1）干冰运输，收到后立即转入液氮冻存或直接复苏；

（2）存活细胞，收到后应继续生长，传代达到细胞生长状态良好时，再进行冻存。具体操作见细胞培养步骤。
细胞用途：仅供科研使用。

注意事项