细胞名称 P815小鼠肥大细胞瘤细胞
Cell Name
描述 小鼠肥大细胞瘤细胞
Description
动物种别 小鼠,DBA/2品系
Organism
组织来源
Tissue and Cell Type
形态 悬浮
Morphology
培养基和添加剂 小鼠肥大细胞瘤细胞P815 完全培养液 配方(100 ml):
Complete Growth Medium and Culture Conditions DMEM (Invitrogen, 12430) 89 ml
FBS (Gibco) 10 ml
Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890 Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031 Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922 Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed:4208429 Ralph P, et al. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem. Biophys. Res. Commun. 61: 1268-1275, 1974. PubMed: 4616699 Lundak RL, Raidt DJ. Cellular immune response against tumor cells. I. In vitro immunization of allogeneic and syngeneic mouse spleen cell suspensions against DBA mastocytoma cells. Cell. Immunol. 9: 60-66, 1973. PubMed: 4270287 Plaut M, et al. Studies on the mechanism of lymphocyte-mediated cytolysis. IV. Specificity of the histamine receptor on effector T cells. J. Immunol. 111: 389-394, 1973. PubMed:4123976 Schmidt W, et al. Cell-free tumor antigen peptide-based cancer vaccines. Proc. Natl. Acad. Sci. USA 94: 3262-3267, 1997. PubMed: 9096381 Gonzalez Armas JC, et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915
运输和保存
可选择干冰运输及发送复苏存活细胞方式:
(1)干冰运输,收到后立即转入液氮冻存或直接复苏;
(2)存活细胞,收到后应继续生长,传代达到细胞生长状态良好时,再进行冻存。具体操作见细胞培养步骤。
细胞用途:仅供科研使用。
注意事项
地址:上海一研生物科技有限公司
Shanghai yiyan bio-technology Co. Ltd.
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