细胞名称 PC-12（未分化）大鼠嗜铬细胞瘤细胞（未分化）
Cell Name
描述 大鼠嗜铬细胞瘤细胞
Description
动物种别 大鼠
Organism
组织来源 肾上腺
Tissue and Cell Type
形态 成团状悬浮、半贴壁；小的形状不规则细胞
Morphology
培养基和添加剂 大鼠嗜铬细胞瘤细胞 完全培养液（100 ml）：
Complete Growth Medium and Culture Conditions RPMI Medium 1640 (Invitrogen, 11875-093) 85 ml
FBS (Gibco) 5 ml
heat-inactivated horse serum (GIBCO, 16050) 10 ml
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed:3839317 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712 Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125 U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

运输和保存

可选择干冰运输及发送复苏存活细胞方式：

（1）干冰运输，收到后立即转入液氮冻存或直接复苏；

（2）存活细胞，收到后应继续生长，传代达到细胞生长状态良好时，再进行冻存。具体操作见细胞培养步骤。
细胞用途：仅供科研使用。

注意事项