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细胞名称 NR8383大鼠肺泡巨噬细胞
Cell Name
描述 NR8383 (正常大鼠，1983年8月3日)来源于肺灌洗时的正常大鼠肺泡巨噬细胞。 细胞在gerbil肺细胞连续培养液存在下培养了大约8-9个月。 随后，不再需要外源生长因子。 通过有限稀释法从单个细胞克隆并亚克隆NR8383细胞，并三次用软琼脂亚克隆。 细胞表现出巨噬细胞的特性，吞噬酵母多糖和铜绿，非特异性脂酶活性，Fc受体，氧化降解；分泌IL-1, TNF beta和IL-6，可重复地响应外源生长因子。 NR8383细胞响应博莱霉素，分泌TGF beta前体。 在博莱霉素刺激下，TGF beta mRNA表达也上升。 细胞对内毒素敏感。 1-10 钠克/毫升的LPS水平抑制增生达50%。 即使达到0.001毫克/毫升的水平，LPS抑制还是无毒且在后续过程中可逆的。 NR8383细胞株提供了高响应的肺泡巨噬细胞的均一来源，可以用于体外研究巨响细胞相关活性。 此细胞半悬浮生长。 在本库通过支原体检测。
Description
动物种别 大鼠
Organism
组织来源 肺；巨噬细胞；肺泡
Tissue and Cell Type
形态 巨噬细胞
Morphology
培养基和添加剂 F12K培养基(SIGMA,货号N3520，添加NaHCO3 2.5g/L)，80%；优质胎牛血清，20%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
Complete Growth Medium and Culture Conditions
供应限制 A。仅供研究之用。
Permits and Restrictions
REFERENCE Hidalgo HA , et al. Pneumocystis carinii induces an oxidative burst in alveolar macrophages. Infect. Immun. 60: 1-7, 1992. PubMed: 1729174 Helmke RJ , et al. A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Dev. Biol. 25: 44-48, 1989. PubMed: 2914814 Helmke RJ , et al. From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell. Dev. Biol. 23: 567-574, 1987. PubMed: 3497918 Limper AH , Standing JE . Vitronectin interacts with Candida albicans and augments organism attachment to the NR8383 macrophage cell line. Immunol. Lett. 42: 139-144, 1994. PubMed: 7534269 Hidalgo HA , et al. The effects of cyclosporine and dexamethasone on an alveolar macrophage cell line (NR8383). Transplantation 53: 620-623, 1992. PubMed: 1549855 Denholm EM , Rollins SM . Expression and secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am. J. Physiol. 264: L36-L42, 1993. PubMed: 7679254 Krieg DP , et al. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect. Immun. 56: 3173-3169, 1988. PubMed: 3141284 Sherman MP , et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191: 1301-1308, 1993. PubMed: 7682068 Griscavage JM , et al. Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329-6337, 1993. PubMed: 7504017 Henderson SA , et al. Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma and lipopolysaccharide. J. Biol. Chem. 269: 25239-25242, 1994. PubMed: 7523382 Huang S , et al. Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts. Biochem. Biophys. Res. Commun. 184: 922-929, 1992. PubMed: 1374243 Hay, R. J., Caputo, J. L, and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227. 1988. Fleming, D.O., Richardson, J. H., TuIis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.
运输和保存
可选择干冰运输及发送复苏存活细胞方式：
（1）干冰运输，收到后立即转入液氮冻存或直接复苏；
（2）存活细胞，收到后应继续生长，传代达到细胞生长状态良好时，再进行冻存。具体操作见细胞培养步骤。
细胞用途：仅供科研使用。
注意事项
1. 收到细胞后，若发现干冰已挥发干净、冻存管瓶盖脱落、破损及细胞有污染，请立即与我们联系。
2. 所有动物细胞均视为有潜在的生物危害性，必须在二级生物安全台内操作，并请注意防护，所有废液及接触过此细胞的器皿需要灭菌后方能丢弃。
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